Regulatory

Part:BBa_K4335012:Design

Designed by: Hang Zhang   Group: iGEM22_UESTC-BioTech   (2022-10-10)


pCrU6.3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
    Illegal NotI site found at 453
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pCrU6.3 was constructed into plasmid <a href="https://parts.igem.org/Part:BBa_K4335038">[pTX2038]</a> <a href="https://parts.igem.org/Part:BBa_K4335040">[pTX2040]</a> as the promoter of gRNA, and was successfully introduced into Chlamydomonas reinhardtii to obtain the positive transformant.


Source

Chlamydomonas reinhardtii

References

[1]Jakab, G., Mougin, A., Kis, M., Pollák, T., Antal, M., Branlant, C., and Solymosy, F. (1997). Chlamydomonas U2, U4 and U6 snRNAs. An evolutionary conserved putative third interaction between U4 and U6 snRNAs which has a counterpart in the U4atac-U6atac snRNA duplex. Biochimie 79: 387–395]

[2] Greiner, A. et al. Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29, 2498–2518 (2017).