Part:BBa_K4335012:Design
pCrU6.3
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 48
Illegal PstI site found at 121 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 48
Illegal PstI site found at 121
Illegal NotI site found at 453 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 48
Illegal PstI site found at 121 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 48
Illegal PstI site found at 121 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
pCrU6.3 was constructed into plasmid <a href="https://parts.igem.org/Part:BBa_K4335038">[pTX2038]</a> <a href="https://parts.igem.org/Part:BBa_K4335040">[pTX2040]</a> as the promoter of gRNA, and was successfully introduced into Chlamydomonas reinhardtii to obtain the positive transformant.
Source
Chlamydomonas reinhardtii
References
[1]Jakab, G., Mougin, A., Kis, M., Pollák, T., Antal, M., Branlant, C., and Solymosy, F. (1997). Chlamydomonas U2, U4 and U6 snRNAs. An evolutionary conserved putative third interaction between U4 and U6 snRNAs which has a counterpart in the U4atac-U6atac snRNA duplex. Biochimie 79: 387–395]
[2] Greiner, A. et al. Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29, 2498–2518 (2017).